Bax (S4) polyclonal antibody

Product Specifications

Background

The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4

Swiss Prot

Q07812

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human Bax.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 21 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Bax (S4) polyclonal antibody detects endogenous levels of Bax protein.

Applications Notes

Western blot (WB) analysis of Bax (S4) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug)