Occludin (A23) polyclonal antibody

Product Specifications

Background

Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by polyunsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

OCLN

Swiss Prot

Q16625

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human Occludin.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 59 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Occludin (A23) polyclonal antibody detects endogenous levels of Occludin protein.

Applications Notes

Western blot (WB) analysis of Occludin (A23) polyclonal antibody at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:The Kidney tissue lysate of Mouse (30ug) Lane4:The Kidney tissue lysate of Rat (30ug)