PTK2 (phospho-S722) polyclonal antibody
Product Specifications
Background
Focal adhesion kinase was initially identified as a major substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK) . FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein-coupled receptors.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1
Swiss Prot
Q05397
Reactivity
Human, Rat
Immunogen
Synthetic phosphopeptide derived from human PTK2 around the phosphorylation site of Serine722.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Tumor Angiogenesis, Contribution of ECM and Cytoskeletal Factors to EMT, G Protein-coupled Receptors Signaling to MAPK Erk
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 125 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PTK2 (phospho-S722) polyclonal antibody detects endogenous levels of PTK2 protein only when phosphorylated at Ser722 .
Applications Notes
Western blot (WB) analysis of PTK2 (phospho-S722) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:EC9706 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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