NKX2-4 polyclonal antibody

Product Specifications

Background

TTF-1 (thyroid transcription factor-1, BCH, BHC, NK-2, Nkx2.1, Nkx2A, TEBP, TTF1) is a member of the Nkx2 family of homeodomain-containing transcription factors and regulates the transcriptional activity of thyroid-specific genes. TTF-1 influences organogenisis and the maintenance of the differentiated phenotypes of various tissues including thyroid, lung and brain. TTF-1, which is present in the epithelium of the lung, regulates transcription of the surfactant proteins (SP) A, B and C and is essential for lung morphogenesis. In the thyroid, TTF-1 elevates the expression of thyroid specific markers, thyroglobulin, thyroperoxidase and thyrotropin receptors. TTF-1 interacts with SRC-1 and CBP in vitro.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Homeobox protein Nkx-2.4; Homeobox protein NK-2 homolog D; NKX2-4; NKX2D

Swiss Prot

Q9H2Z4

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human NKX2-4.

Conjugation

Unconjugated

Applications

Dilution

WB:1:500~1:1000

Purity

The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NKX2-4 polyclonal antibody detects endogenous levels of NKX2-4 protein.

Applications Notes

Western blot (WB) analysis of NKX2-4 polyclonal antibody at 1:500 dilution LaneA:MCF-7 whole cell lysate LaneB:H1792 whole cell lysate LaneC:PC12 whole cell lysate LaneD:CT-26 whole cell lysate