CTBP1 polyclonal antibody
Product Specifications
Background
CtBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CtBP1 and the related protein CtBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T-cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1 induced transcriptional silencing. In addition, CtBP complexes with CtIP, a protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. CtIP/CtBP binding to BRCA1 inhibits the transactivation of the p21 promoter, and it is critical for regulating p21 transcription in response to DNA damage.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
C-terminal-binding protein 1; CtBP1; CTBP1; CTBP
Swiss Prot
Q13363
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human CTBP1.
Conjugation
Unconjugated
Related Pathways
Notch Signaling
Applications
WB IHC IF
Dilution
WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CTBP1 polyclonal antibody detects endogenous levels of CTBP1 protein.
Applications Notes
Western blot (WB) analysis of CTBP1 polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Hela whole cell lysate LaneC:H1688 whole cell lysate LaneD:The Lung tissue lysate of Mouse LaneE:The Kidney tissue lysate of Mouse LaneF:The Lung tissue lysate of Rat LaneG:The Kidney tissue lysate of Rat
Host or Source
Rabbit
Available Sizes
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