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Raptor (phospho-Ser792) polyclonal antibody

Product Specifications

Background

Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR) -scaffold protein; RPTOR; KIAA1303; RAPTOR

Swiss Prot

Q8N122

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human Raptor around the phosphorylation site of Ser792.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Insulin &Glucose Signaling, Glutamine Metabolism, MTOR signaling

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 150 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

A phospho specific peptide corresponding to residues surrounding Ser792 of human RPTOR

Applications Notes

Western blot analysis of extracts of 293T cells, using Phospho-Raptor-Ser792 antibody at 1:1000 dilution.293T cells were treated by Oligomycin (0.5 uM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25ug per lane.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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