Gab2 (Ab-623) polyclonal antibody

Product Specifications

Background

GRB2 associated binding protein 2 (GAB2) Homo sapiens This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

GAB2; KIAA0571; GRB2-associated-binding protein 2; GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; pp100

Swiss Prot

Q9UQC2

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Gab2 around the phosphorylation site of Tyr643. AA range:609-658

Conjugation

Unconjugated

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Unmodification

Molecular Weight

~ 74 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For

Specificity

Phospho-Gab 2 (Y643) Polyclonal Antibody detects endogenous levels of Gab 2 protein only when phosphorylated at Y643.

Applications Notes

Immunohistochemistry analysis of paraffin-embedded human brain, using Gab2 (Phospho-Tyr643) Antibody. The picture on the right is blocked with the phospho peptide.