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PCNA monoclonal antibody

Product Specifications

Background

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

Proliferating cell nuclear antigen; PCNA; Cyclin

Swiss Prot

P12004

Reactivity

Human, Mouse, Rat, Monkey, Hamster

Immunogen

Purified recombinant human PCNA protein fragments expressed in E.coli.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, IP

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of PCNA protein.

Applications Notes

Western blot analysis of PCNA expression in Hela (A), NIH3T3 (B), COS7 (C), C6 (D), CHOK1 (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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