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CaMK4 monoclonal antibody

Product Specifications

Background

CaMKIV is an important member of calcium/calmodulin-activated kinases. CaMKIV signaling has been related to long-term neural potentiation and memory, as well as T-cell receptor signaling . CaMKIV has catalytic and regulatory domains. The Ca2+/calmodulin-dependent CaMKK phosphorylates CaMKIV, releasing the autoinhibitory effect and thus activating the kinase. The activated CaMKIV further autophosphorylates itself at Thr196 (or Thr200 in human) to render the kinase constitutively active . The threonine phosphorylation state of CaMKIV can be downregulated by PP2A dephosphorylation.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

CAMK; CAMK-GR; CAMKIV; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; CaM kinase-GR

Swiss Prot

Q16566

Reactivity

Human

Immunogen

Purified recombinant human CaMKIV protein fragments expressed in E.coli.

Conjugation

Unconjugated

Related Pathways

Death Receptor Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Regulation of Microtubule Dynamics, T cell receptor signaling

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of CaMK4 protein.

Applications Notes

Western blot analysis of CaMK4 expression in Romas (A), Jurkat (B) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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