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Phospho-ERK1/2 (Thr202/Thr185) monoclonal antibody

Product Specifications

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

CAS Number

9007-83-4

Structure Composition

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Product Name Alternative

MAPK1/MAPK3

Swiss Prot

P27361/P28482

Reactivity

Human, Hamster, Rat

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr185 of human ERK2

Conjugation

Unconjugated

Applications

WB, IP

Dilution

WB: 1/500-1/1000 IP: 1/20

Purity

Affinity Purified

Modification

Phosphorylated

Molecular Weight

Calculated MW: 44,42 kDa; Observed MW: 44,42 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Phospho-ERK1 /ERK2 in Hela, Hela+TPA lysates using Phospho-ERK1/2 antibody.

Host or Source

Rabbit

Available Sizes

Curated Selection

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