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Acetyl-Histone H3 (Lys9) polyclonal antibody

Product Specifications

Background

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

H3K9ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

Swiss Prot

P68431

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys9. AA range:3-52

Conjugation

Unconjugated

Applications

WB, IHC-F, IHC-P, ICC/IF, ELISA

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purity

Affinity Purified

Modification

Acetylated

Molecular Weight

Calculated MW: 15 kDa; Observed MW: 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot analysis of Acetyl-Histone H3 in COS7 and C6 lysates using Acetyl-Histone H3 antibody.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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