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Recombinant Carassiusauratus Leptin Protein (His Tag)

Product Specifications

Background

Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish.

Abbreviation

Leptin

Synonyms

LEP; Leptin; OB; OBS; Obese Protein; Obesity Factor

UniProt

B8YI02

Accession Number

B8YI02

Expression System

P.Pichia

Tag

N-His

Sequence

Pro22-Cys171

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.3 kDa

Observed Molecular Weight

17 kDa

Species

Carassius auratus

Available Sizes

Frequently Asked Questions

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