Smad2/3 (S2) polyclonal antibody

Product Specifications

Background

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Swiss Prot

P84022

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human Smad2/3.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Contribution of Soluble Factors to EMT, hippo Signaling, TGFβ Smad signaling

Applications

WB IP IF

Dilution

WB: 1:1000~1:2000 IF 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 52, 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Smad2/3 (S2) pAb detects endogenous levels of Smad2/3 protein.

Applications Notes

Western blot (WB) analysis of Smad2/3 (S2) pAb at 1:1000 dilution Lane1:PC12 whole cell lysate (30ug) Lane2:CT-26 whole cell lysate (30ug) Lane3:Hela whole cell lysate (30ug) Lane4:HEK293T whole cell lysate (30ug) Lane5:EC9706 whole cell lysate (30ug)