DGKA mouse monoclonal antibody

Product Specifications

Background

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

CAS Number

9007-83-4

Structure Composition

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Product Name Alternative

SSTNI; TNN1

Swiss Prot

P19237

Reactivity

Human

Immunogen

Full length human recombinant protein of human TNNI1 (NP_003272) produced in E.coli.

Conjugation

Unconjugated

Applications

WB, IHC

Dilution

WB 1:500, IHC 1:150

Purity

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Modification

Unmodified

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DGKA mouse monoclonal antibody detects endogenous levels of DGKA protein.

Applications Notes

Western blot (WB) analysis of DGKA mouse monoclonal antibody at 1:500 dilution Lane1:EC9706 whole cell lysate (30ug) Lane2:HCT116 whole cell lysate (30ug)