MRP-S25 (K168) polyclonal antibody

Product Specifications

Background

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-S25 (mitochondrial 28S ribosomal protein S25), also known as S25mt, is a 173 amino acid mitochondrial ribosomal protein belonging to the ribosomal protein S25/L51 family. Localized to mitochondria, MRP-S25 is present in the 28S subunit of the mitoribosomes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

28S ribosomal protein S25, mitochondrial; MRP-S25; S25mt; MRPS25; RPMS25

Swiss Prot

P82663

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 140-180 of Human MRP-S25.

Conjugation

Unconjugated

Applications

WB IP

Dilution

WB: 1:500~1:1000 IP: 1:10-100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MRP-S25 (K168) polyclonal antibody detects endogenous levels of MRP-S25 protein.

Applications Notes

Western blot (WB) analysis of MRP-S25 (K168) pAb at 1:1000 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (20ug) Lane4:HEK293T whole cell lysate (20ug) Lane5:HCT116 whole cell lysate (20ug)