Beclin 1 polyclonal antibody
Product Specifications
Background
BECN1 (beclin 1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the ribosomal protein S6, and both are mediated via amino acids. BECN1 expression in various carcinoma cell lines, such as MCF7, is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis and, subsequently, inhibits cellular proliferation. Additionally, BECN1 shares structural similarity to the yeast autophagy gene product, APG6, and was one of the first mammalian proteins discovered to mediate autophagy.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
APG6; ATG 6; ATG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; Beclin 1 (coiled coil moesin like BCL2 interacting protein) ; Beclin 1 autophagy related; Beclin-1; Beclin1; BECN 1; BECN1; BECN1; Coiled coil myosin like BCL2 interacting protein; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197 antibod
Swiss Prot
Q14457
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fragment corresponding to a region within amino acids 141-451 of Human Beclin 1.
Conjugation
Unconjugated
Related Pathways
Autophagy Signaling
Applications
WB IHC IF
Dilution
WB: 1:2000~1:5000 IHC: 1:50~1:200 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Beclin 1 polyclonal antibody detects endogenous levels of Beclin 1 protein.
Applications Notes
Western blot (WB) analysis of Beclin 1 pAb at 1:2000 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:PMVEC whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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