P53 (phospho-S15) polyclonal antibody
Product Specifications
Background
P53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53
Swiss Prot
P04637
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human p53 around the phosphorylation site of Serine 15.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Hypoxia Signaling, Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 53 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-p53 (S15) polyclonal antibody detects endogenous levels of p53 protein only when phosphorylated at Ser15
Applications Notes
Western blot (WB) analysis of p-p53 (S15) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with colchicine (0.2ng/ml,24h) Lane2:Raw264.7 cell lysate treated with colchicine (0.2ng/ml,24h) Lane3:H9C2 cell lysate treated with colchicine (0.2ng/ml,24h)
Host or Source
Rabbit
Available Sizes
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