BOB-1 Antibody
POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB-1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D) J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
Q64693
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of mouse BOB-1 (EEEESNTYELNHTLSVEGF) was used as the immunogen for this BOB-1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Limitations
This BOB-1 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Image Legend
Western blot testing of BOB-1 antibody and Lane 1: rat spleen tissue lysate. Predicted molecular weight: ~28/35-40kDa (unmodified/ubiquitinated) .
Frequently Asked Questions
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