Anti-Interleukin-10 (IL-10)
Rabbit polyclonal antibody to Interleukin-10 (IL10)
Product Specifications
Reactivity
Human
Immunogen
Recombinant (Human) full length protein produced in E. coli
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Affinity purified
Dilution
ELISA: 1/400 - 1/1200 (as detection antibody, using a monoclonal antibody for capture) . IHC-Fr: 1/400. IHC-P: 1/400. IP: 1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background. Neut: 1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. RIA: 1/4000. WB: 1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule
Purity
Affinity purified
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: 1/400 - 1/1200 (as detection antibody, using a monoclonal antibody for capture) . IHC-Fr: 1/400. IHC-P: 1/400. IP: 1/400 - 1/800. Pre-clearing with a non-specific rabbit IgG is recommended to reduce background. Neut: 1/400. Incubate the samples with the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. RIA: 1/4000. WB: 1/400 - 1/800. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule
Tested Applications
ELISA, ICC, IF, IHC, IP, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% Sodium Azide Constituents: Whole serum
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