IP-10, Human, mAb 6D4 Antibody
Mouse monoclonal antibody to IP-10
Product Specifications
Product Name Alternative
10 kDa interferon gamma-induced protein, CXCL10, Small-inducible cytokine B10 Gene name: CXCL10
Reactivity
Human
Immunogen
Recombinant human IP-10
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
FC: Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3) W: Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4) . IHC-F: Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As positive control chronically inflamed liver was used and as negative control normal liver and isotype matched antibody (Ref.4) .
Tested Applications
FC, IA, IF, IHC-Fr, WB
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Mouse IgG2a
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