TFE3 Antibody / Transcription factor E3

Transcription factor E3 (TFE3) belongs to the basic helix-loop-helix zipper transcription factor family. The TFE3 gene is located on the short arm of the X chromosome, is expressed in various cells of the human body, and participates in the regulation of various genes. The rearrangement of this gene is associated with a variety of tumors, and it is highly expressed in acinar soft tissue sarcoma with TFE3 fusion gene, Xp11.2 translocation/TFE3 gene fusion-related renal cell carcinoma, and in perivascular epithelioid cell tumors. positive expression.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P19532

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human TFE3 protein was used as the immunogen for the TFE3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

TFE3/6849R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the TFE3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TFE3 antibody is available for research use only.

Storage Conditions

Aliquot the TFE3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the TFE3 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human MiT translocation RCC with TFE3 antibody. Nuclear staining using TFE3/6849R mAb at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.