Octamer-binding protein 4 Antibody / OCT4

OCT4 (Octamer-binding protein 4) is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q01860

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant fragment of the human protein was used as the immunogen for the Octamer-binding protein 4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

OCT4/7347R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Octamer-binding protein 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Octamer-binding protein 4 antibody is available for research use only.

Storage Conditions

Aliquot the Octamer-binding protein 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Octamer-binding protein 4 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human seminoma tissue with Octamer-binding protein 4 antibody (clone OCT4/7347R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.