Sarcomeric Actinin Alpha 2 Antibody / ACTN2

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P35609

Host

Rabbit

Reactivity

Human

Immunogen

A portin of amino acids 557-692 from the human protein was used as the immunogen for the Sarcomeric Actinin Alpha 2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ACTN2/8184R

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Sarcomeric Actinin Alpha 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Sarcomeric Actinin Alpha 2 antibody is available for research use only.

Storage Conditions

Aliquot the Sarcomeric Actinin Alpha 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Sarcomeric Actinin Alpha 2 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human skeletal muscle tissue with ACTN2 antibody (clone ACTN2/8184R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.