S100 calcium-binding protein A13 Antibody / S100A13

The S-100 protein family consists of a group of calcium-binding proteins, that exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A13 is a 98 amino acid protein with 2 EF-hand calcium-binding domains. High levels of S-100A13 mRNA are present in skeletal muscle, heart, kidney, ovary, small intestine and pancreas. S-100A13 translocates in response to elevated intracellular calcium levels induced by Angiotensin II. S-100A13 co-localizes with S-100A1 on human chromosome 1q21.3, the site where the majority of S-100 proteins cluster.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q99584

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-93) from the human protein was used as the immunogen for the S100 calcium-binding protein A13 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

S100A13/7482

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the S100 calcium-binding protein A13 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This S100 calcium-binding protein A13 antibody is available for research use only.

Storage Conditions

Aliquot the S100 calcium-binding protein A13 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the S100 calcium-binding protein A13 antibody should be determined by the researcher.

Location

Cytoplasm, Nuclear, Plasma membrane, Secreted

Image Legend

IHC staining of FFPE human spleen tissue with S100 calcium-binding protein A13 antibody (clone S100A13/7482) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.