GCHFR Antibody / GFRP

GTP cyclohydrolase I feedback regulatory protein (GFRP) is encoded by the gene GCHFR. GFRP mediates feedback inhibition of GTP cyclohydrolase I activity by tetrahydrobiopterin. GFRP also acts as a mediator for the stimulatory effect of phenylalanine on enzyme activity. L-phenylalanine reverses this inhibition. Cross-linking experiments have shown that GFRP is usually expressed as a homodimer or pentamer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P30047

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human GCHFR protein was used as the immunogen for the GCHFR antibody.

Clonality

Monoclonal

Isotype

IgG2 κ

Clone

GCHFR/7734

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the GCHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GCHFR antibody is available for research use only.

Storage Conditions

Aliquot the GCHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the GCHFR antibody should be determined by the researcher.

Location

Lysosome

Image Legend

IHC staining of FFPE human liver tissue with GCHFR antibody (clone GCHFR/7734) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.