Biotin Antibody
This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Product Specifications
CAS Number
9000-83-3
Specifications
UniProt
Not Applicable
Host
Rabbit
Reactivity
All Species Expected
Immunogen
Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
BTN/8672R
Type
Recombinant
Applications
FACS, IF, WB, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Biotin antibody is available for research use only.
Storage Conditions
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Biotin antibody should be determined by the researcher.
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