NFkB-p105 (A901) polyclonal antibody
Product Specifications
Background
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light; polypeptide gene enhancer in B-cells 1; NFKB1; NFkB p105
Swiss Prot
P19838
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 920-970 of Human NFkB-p105.
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, STING Signaling, Inhibition of Apoptosis
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 105 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NFkB-p105 (A901) polyclonal antibody detects endogenous levels of NFκB-p105 protein.This antibody does not recognize NFkB-p50 subunit.
Applications Notes
Western blot (WB) analysis of NFkB-p105 (A901) polyclonal antibody at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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