FCR1

FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max emissions at 525nm. Treatment with a mild reducing agent (including sodium cyanoborohydride, DTT, and glutathione) reduces the flavin thereby decreasing the green fluorescence intensity, and increasing the blue. Re-oxidation can be achieved using mild oxidizing agents. In general, the FCR1 probe can be used to observe changes in oxidative capacity without interference from background effects.

7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1)