Choline Acetyltransferase Antibody: RPE

Rabbit Anti-Human Choline Acetyltransferase Polyclonal

Product Specifications

Background

Choline acetyltransferase (ChAT) catalyzes the synthesis of acetylcholine (ACh), a neurotransmitter essential for motor control, memory, and autonomic function. ChAT is selectively expressed in cholinergic neurons, making it a reliable marker for cholinergic system integrity. Loss of ChAT activity is a hallmark of several neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and Down syndrome. Reduced ChAT expression correlates with cognitive decline and impaired synaptic transmission in affected brain regions. As a neuron-specific enzyme with a direct role in neurotransmitter synthesis, ChAT is both a diagnostic marker and a therapeutic target in disorders characterized by cholinergic dysfunction.

CAS Number

9007-83-4

Specifications

Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.

Product Name Alternative

CHAT, Choline acetyltransferase, Acetyl-CoA choline O-acetyltransferase, Choline acetylase, CLAT_HUMAN, CMS1A

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

1103

Swiss Prot

P28329

Accession Number

NP_001136401.1

Cellular Locus

Cytoplasm | Cytosol | Mitochondrion | Nucleus

Host

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase

Target

Choline Acetyltransferase

Clonality

Polyclonal

Conjugation

RPE

Type

Polyclonal

Applications

WB | ICC/IF

Validated Applications

WB,IHC,ICC/IF

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-706 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266 (1) : 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.