Human Fatty acid-binding protein, heart (FABP3) ELISA Kit

Heart fatty acid binding protein (hFABP) is a novel small cytosolic protein that is abundant in the heart. It is highly cardiac-specific, but is also expressed at low concentrations in tissues outside the heart. After myocardial ischemic damage, hFABP can be detected in the blood as early as 1-3 h after onset of chest pain, with peak values reached at 6-8 h and plasma levels returning to normal within 24-30 h. The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells.

FABP3; FABP11; H-FABP; MDGI; O-FABP; Fatty acid-binding protein 3; muscle; fatty acid binding protein 11; fatty acid binding protein 3; mammary-derived growth inhibitor

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Fatty acid-binding protein, heart (FABP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human FABP3. No significant cross-reactivity or interference between Human FABP3 and analogues was observed.

This Human Fatty acid-binding protein, heart (FABP3) ELISA Kit employs a two-site sandwich ELISA to quantitate FABP3 in samples. An antibody specific for FABP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFABP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

FABP3; FABP11; H-FABP; MDGI; O-FABP; Fatty acid-binding protein 3; muscle; fatty acid binding protein 11; fatty acid binding protein 3; mammary-derived growth inhibitor

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.