Human Testis anion transporter 1 (SLC26A8) ELISA Kit

SLC26A8 is one member of a family of sulfate/anion transporter genes. Family members are well conserved in their genomic (number and size of exons) and protein (aa length among species) structures yet have markedly different tissue expression patterns. This gene is expressed primarily in spermatocytes. Two transcript variants encoding different isoforms have been found. The deduced 970-amino acid protein has a calculated molecular mass of 109 kD. SLC26A8 contains 14 putative transmembrane regions with cytoplasmic N- and C-termini. It also contains several N-glycosylation sites, 4 of which reside in the major predicted external loop. SLC26A8 shares 26% identity with pendrin (SLC26A4), and 28% identity with DRA (SLC26A3) .

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Human Testis anion transporter 1 (SLC26A8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC26A8. No significant cross-reactivity or interference between Human SLC26A8 and analogues was observed.

This Human Testis anion transporter 1 (SLC26A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC26A8 in samples. An antibody specific for SLC26A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC26A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC26A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC26A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.