Rho GDIα (phospho Ser174) Polyclonal Antibody

Rabbit Anti-Rho GDIα (phospho Ser174) Polyclonal Antibody

Product Specifications

Background

ARHGDIA encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

ARHGDIA; GDIA1; Rho GDP-dissociation inhibitor 1; Rho GDI 1; Rho-GDI alpha

Gene ID

396

Accession Number

P52565

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Rho GDIα around the phosphorylation site of S174

Clonality

Polyclonal

Tag

Rho GDP-dissociation inhibitor 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

23kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Rho GDIα (S174) Polyclonal Antibody detects endogenous levels of Rho GDIα protein only when phosphorylated at S174.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200