Ep-CAM Antibody

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P16422

Host

Rabbit

Reactivity

Human

Immunogen

This Ep-CAM antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 299-334 amino acids from the C-terminal region of the human protein.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Ep-CAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Ep-CAM antibody is available for research use only.

Storage Conditions

Aliquot the Ep-CAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Ep-CAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig

Image Legend

Western blot analysis of lysate from A431, HT-29 cell line (left to right) using Ep-CAM antibody diluted at 1:1000 for each lane. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated) .