C/EBP ε (phospho Thr74) Polyclonal Antibody

Rabbit Anti-C/EBP ε (phospho Thr74) Polyclonal Antibody

Product Specifications

Background

The CCAAT/enhancer binding protein epsilon encoded by CEBPE is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.

CAS Number

9007-83-4

Product Name Alternative

CEBPE; CCAAT/enhancer-binding protein epsilon; C/EBP epsilon

Gene ID

1053

Accession Number

Q15744

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human C/EBP ε around the phosphorylation site of T74

Clonality

Polyclonal

Tag

CCAAT enhancer-binding protein epsilon

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

34kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-C/EBP ε (T74) Polyclonal Antibody detects endogenous levels of C/EBP ε protein only when phosphorylated at T74.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.