AChRα3 Polyclonal Antibody

Rabbit Anti-AChRα3 Polyclonal Antibody

Product Specifications

Background

CHRNA3 encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. CHRNA3 encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein (cholinergic receptor nicotinic alpha 3 subunit) is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

CAS Number

9007-83-4

Product Name Alternative

CHRNA3; NACHRA3; Neuronal acetylcholine receptor subunit alpha-3

Gene ID

1136

Accession Number

P32297

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human AChRα3

Clonality

Polyclonal

Tag

Neuronal acetylcholine receptor subunit alpha-3

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

57kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

AChRα3 Polyclonal Antibody detects endogenous levels of AChRα3 protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.