Cyclin B1 Mouse Monoclonal Antibody (1A5)

Mouse Anti-Cyclin B1 Mouse Monoclonal Antibody (1A5)

Product Specifications

Background

The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

CAS Number

9007-83-4

Product Name Alternative

CCNB1

Gene ID

891

Accession Number

P14635

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic Peptide of Cyclin B1 at AA range of 60-140

Clonality

Monoclonal

Tag

CCNB1

Applications

IHC, IF

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Molecular Weight

55kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Cyclin B1 protein detects endogenous levels of CCNB1.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-200; IF 1:50-200