NFκB-p65 (phospho Thr435) rabbit pAb

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Product Specifications

Background

UniProt

Q04206

Swiss Prot

Q04206

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr435. AA range:402-451

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; IP; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

60kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

60kD

Fragment

IgG

Subcellular Location

Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333) . Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135) . Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752) . Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622) . .