Pdcd-4 (phospho Ser457) rabbit pAb

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010],

Product Specifications

Background

Product Name Alternative

PDCD4; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a

UniProt

Q53EL6

Swiss Prot

Q53EL6

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human PDCD4 around the phosphorylation site of Ser457. AA range:420-469

Target

PDCD4

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

51kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

51kD

Fragment

IgG

Subcellular Location

Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm (By similarity) . Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133) . .