FTO rabbit pAb

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011],

Product Specifications

Background

Product Name Alternative

FTO; KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein

UniProt

Q9C0B1

Swiss Prot

Q9C0B1

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human FTO. AA range:19-68

Target

FTO

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

58kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

58kD

Fragment

IgG

Subcellular Location

Nucleus . Nucleus speckle . Cytoplasm . Localizes mainly in the nucleus, where it is able to demethylate N (6) -methyladenosine (m6A) and N (6),2'-O-dimethyladenosine cap (m6A (m) ) in U6 small nuclear RNA (snRNA), N (1) -methyladenine from tRNAs and internal m6A in mRNAs (PubMed:30197295) . In the cytoplasm, mediates demethylation of m6A and m6A (m) in mRNAs and N (1) -methyladenine from tRNAs (PubMed:30197295) . .