Pyruvate Oxidase from Aerococcus sp.

<strong>Pyruvate Oxidase from Aerococcus sp.</strong>_x000D_ <strong>Catalog number:</strong> B2017855_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 25 Units_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Powder_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -20°C_x000D_ <strong>Keywords:</strong> Pyruvate: oxygen oxidoreductase (phosphorylating)_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Lu J, Zhao Y, Zhang J. High-level expression of Aerococcus viridans pyruvate oxidase in Escherichia coli by optimization of vectors and induction conditions Lett Appl Microbiol. 2018 Sep;67(3):262-269._x000D_ 2: Juan EC, Hoque MM, Hossain MT, Yamamoto T, Imamura S, Suzuki K, Sekiguchi T, Takénaka A. The structures of pyruvate oxidase from Aerococcus viridans with cofactors and with a reaction intermediate reveal the flexibility of the active-site tunnel for catalysis Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt 11):900-7._x000D_ 3: Ashok Y, Maksimainen MM, Kallio T, Kilpeläinen P, Lehtiö L. FMN-dependent oligomerization of putative lactate oxidase from Pediococcus acidilactici PLoS One. 2020 Feb 24;15(2):e0223870._x000D_ 4: Lu J, Zhang J. Extracellular expression of Aerococcus viridans pyruvate oxidase in recombinant Escherichia coli through SecB co-expression RSC Adv. 2019 Aug 21;9(45):26291-26301._x000D_ 5: Nakamura H, Tanaka H, Hasegawa M, Masuda Y, Arikawa Y, Nomura Y, Ikebukuro K, Karube I. An automatic flow-injection analysis system for determining phosphate ion in river water using pyruvate oxidase G (from Aerococcus viridans) Talanta. 1999 Nov 15;50(4):799-807._x000D_ 6: Li SJ, Umena Y, Yorita K, Matsuoka T, Kita A, Fukui K, Morimoto Y. Crystallographic study on the interaction of L-lactate oxidase with pyruvate at 1.9 Angstrom resolution Biochem Biophys Res Commun. 2007 Jul 13;358(4):1002-7._x000D_ 7: Streitenberger SA, López-Más JA, Sánchez-Ferrer A, García-Carmona F. Use of dye affinity chromatography for the purification of Aerococcus viridans lactate oxidase Biotechnol Prog. 2002 May-Jun;18(3):657-9._x000D_ 8: Leiros I, Wang E, Rasmussen T, Oksanen E, Repo H, Petersen SB, Heikinheimo P, Hough E. The 2.1 A structure of Aerococcus viridans L-lactate oxidase (LOX) Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt 12):1185-90._x000D_ 9: Stoisser T, Klimacek M, Wilson DK, Nidetzky B. Speeding up the product release: a second-sphere contribution from Tyr191 to the reactivity of L-lactate oxidase revealed in crystallographic and kinetic studies of site-directed variants FEBS J. 2015 Nov;282(21):4130-40._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/29355461">10: Liang J, Zhao J, Wang Z, Wang Y. Temperature gradient-based high-cell density fed-batch fermentation for the production of pyruvate oxidase by recombinant E. coli Prep Biochem Biotechnol. 2018 Feb 7;48(2):188-193. </a>_x000D_ _x000D_ <strong>Products Related to Pyruvate Oxidase from Aerococcus sp. can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>