Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Recombinant Rabbit Monoclonal Antibody [JE57-42]
Selected Variant
HA722146
Size: 100 µL
Serine/threonine-protein kinase PAK 4 is an enzyme that in humans is encoded by the PAK4 gene. Serine/threonine-protein kinase PAK 5 is an enzyme that in humans is encoded by the PAK5 gene. Serine/threonine-protein kinase PAK 6 is an enzyme that in humans is encoded by the PAK6 gene. PAK4 is one of six members of the PAK family of serine/threonine kinases which are divided into group I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK6 and PAK5/7). PAK4 localizes in sub-cellular domains of the cytoplasm and nucleus. PAK4 regulates cytoskeleton remodeling, phenotypic signaling and gene expression, and affects directional motility, invasion, metastasis, and growth. Similar to PAK1, PAK4-signaling-dependent cellular functions also regulate both physiologic and disease processes such as cancer, as PAK4 is overexpressed and/or hyperstimulated in human cancer, at-large. The PAK5 enzyme is one of three members of Group II PAK family of serine/threonine kinases, and evolutionary conserved across species. PAK6 belongs to the Group II PAK family members. Like other family members, PAK6 is also evolutionary conserved across species. Phospho-PAK4+PAK5+PAK6 (S474+S560+S602) Antibody detects endogenous levels of PAK4, PAK5 and PAK6 only when phosphorylated at serine 474, 602, or 560, respectively.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
PAK4, KIAA1142, PAK5, KIAA1264, PAK7, PAK6
Swiss Prot
O96013 Human, Q9P286 Human, Q9NQU5 Human
Cellular Locus
Cytoplasm; Mitochondrion, Cytoplasm, Nucleus; Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4.
Isotype
IgG
Clone
JE57-42
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
A549 cell lysate, HeLa cell lysate, MCF7 cell lysate, HUVEC cell lysate, HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 64/81/75 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Price
Frequently Asked Questions
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