Anti-SUR1 Antibody

Mouse Monoclonal Antibody specific to SUR1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Sulfonylurea receptor 1

Gene Name

Abcc8

NCBI Gene ID

UniProt

Q09429

Accession Number

NP_037171.2

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1548-1582 (cytoplasmic C-terminus) of rat SUR1 (accession no. NP_037171.2).

Target Antigen

ATP-binding cassette sub-family C member 8

Target

SUR1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release. {UniProtKB:Q09428}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~160kDa is detected. Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Mouse brain membranes.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat SUR1. It does not cross-react with SUR2B.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Sulfonylurea receptors (SUR) are membrane proteins that are molecular targets of the sulfonylurea class of antidiabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the SUR is to sense intracellular levels of ATP and ADP and facilitate the opening or closing of its associatedKir6.x potassium channel, thus monitoring the energy balance within cells.