Recombinant Human CASP9 Protein (Sumo Tag)

Product Specifications

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7) . Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) .

Abbreviation

CASP9

Synonyms

MCH; Apoptotic protease Mch; PPP1R; ICE-LAP; CASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9; Caspase 9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3 (APAF-3) ; ICE-like apoptotic protease 6 (ICE-LAP6) ; Apoptosis related cysteine peptidase; Apoptotic protease-activating factor 3; Caspase 9 apoptosis related cysteine peptidase; Caspase 9 Dominant Negative; Caspase 9c; Caspase-9 subunit p10; ICE LAP6; ICE like apoptotic protease 6; ICE-like apoptotic protease 6; protein phosphatase 1; regulatory subunit 56; RNCASP9

UniProt

P55211

Accession Number

P55211

Expression System

E.coli

Tag

N-Sumo

Sequence

Val139-Asp330

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.