Recombinant Mouse NT-proBNP Protein (Trx Tag)
Product Specifications
Background
Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from the ventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.
Abbreviation
NT-proBNP
Synonyms
B-Type Natriuretic Peptide; GC-B; Natriuretic Peptide Precursor B; NT-Pro-BNP; Nppb
UniProt
P40753
Accession Number
P40753
Expression System
E.coli
Tag
N-Trx
Sequence
Ser26-Arg76
Field of Research
Cardiovascular; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
25.5 kDa
Observed Molecular Weight
30 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Mouse
Available Sizes
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