Human Granulocyte Colony-Stimulating Factor Receptor, CSF3R ELISA Kit

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3) . It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin (Ig) -like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML) . Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3200 pg/ml | 2.387 | 2.31 | 2.207 2.233 1600 pg/ml | 1.335 | 1.269 | 1.166 1.204 800 pg/ml | 0.749 | 0.702 | 0.599 0.656 400 pg/ml | 0.38 | 0.381 | 0.278 0.383 200 pg/ml | 0.238 | 0.24 | 0.137 0.243 100 pg/ml | 0.18 | 0.168 | 0.065 0.157 50 pg/ml | 0.139 | 0.134 | 0.031 0.13 0 pg/ml | 0.106 | 0.103 | 0 0.101

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 333 | 22.6 | 6.8 Intra-assay | 2 | 20 | 679 | 24.1 | 3.5 Intra-assay | 3 | 20 | 1997 | 96.8 | 4.8 Inter-assay | 1 | 20 | 335 | 20.5 | 6.1 Inter-assay | 2 | 20 | 671 | 33.6 | 5 Inter-assay | 3 | 20 | 1990 | 110 | 5.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail