Human Interleukin-18, IL18 ELISA Kit

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.74 | 2.84 | 2.722 2.94 1250 pg/ml | 1.427 | 1.448 | 1.33 1.469 625 pg/ml | 0.758 | 0.753 | 0.635 0.748 312.5 pg/ml | 0.438 | 0.433 | 0.315 0.428 156.25 pg/ml | 0.276 | 0.266 | 0.148 0.256 78.125 pg/ml | 0.176 | 0.189 | 0.071 0.202 39.0625 pg/ml | 0.141 | 0.145 | 0.027 0.149 0 pg/ml | 0.117 | 0.118 | 0 0.119

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 163 | 6.8 | 4.2 Intra-assay | 2 | 20 | 494 | 19.2 | 3.9 Intra-assay | 3 | 20 | 1766 | 65.9 | 3.7 Inter-assay | 1 | 20 | 162 | 21.3 | 13.1 Inter-assay | 2 | 20 | 493 | 48.4 | 9.8 Inter-assay | 3 | 20 | 1761 | 150 | 8.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail