Supreme TC Grade Agar

Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium. The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.

Product Specifications

CAS Number

9002-18-0

Synonyms

Gelose, Agar Agar Agar

HS Code

1302.31

Applications

Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.

Assay Protocol

Follow the given steps to prepare 1 liter of tissue culture media using PCT’s Supreme TC Agar: Take a beaker and add 800 ml of distilled water to it. To the water add 4.54 grams of PCT’s MS Media (or alternative medium based on your plant), 30 grams of sugar, and PPM™ (generally 1-2 ml/L). Make up the volume of the media to 1000ml. Adjust the pH of the media between 5.4-5.8. Add 6-8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring. Autoclave the medium for 20 minutes at 15 psi and 121°C.