Influenza A H1N1 (Swine Flu 2009) Hemagglutinin ELISA Kit

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.569 | 2.458 | 2.346 2.348 1000 pg/ml | 1.344 | 1.328 | 1.216 1.312 500 pg/ml | 0.732 | 0.719 | 0.607 0.707 250 pg/ml | 0.416 | 0.422 | 0.31 0.429 125 pg/ml | 0.267 | 0.268 | 0.156 0.27 62.5 pg/ml | 0.186 | 0.192 | 0.08 0.199 31.25 pg/ml | 0.149 | 0.151 | 0.039 0.154 0 pg/ml | 0.115 | 0.112 | 0 0.109

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 140 | 4.7 | 3.4 Intra-assay | 2 | 20 | 368 | 12.2 | 3.3 Intra-assay | 3 | 20 | 1151 | 39.8 | 3.5 Inter-assay | 1 | 20 | 141 | 14.4 | 10.2 Inter-assay | 2 | 20 | 371 | 27.3 | 7.4 Inter-assay | 3 | 20 | 1160 | 81.2 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail