Mouse Prostasin ELISA Kit PicoKine®

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1504 1621 1563 1509 1549 47.44 3% Sample 2 6502 6771 6290 6265 6457 203.33 3.1% Sample 3 26206 23293 23327 24983 24452 1221.41 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1504 1621 1563 1509 1549 47.44 3% Sample 1 1504 1621 1563 1509 1549 47.44 3% Sample 2 6502 6771 6290 6265 6457 203.33 3.1% Sample 2 6502 6771 6290 6265 6457 203.33 3.1% Sample 3 26206 23293 23327 24983 24452 1221.41 4.9% Sample 3 26206 23293 23327 24983 24452 1221.41 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Standard deviation 102.27 436.7%3 1205.47 113.78 484.7 1723.11 CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Standard deviation 102.27 436.7%3 1205.47 113.78 484.7 1723.11 CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Mean (pg/ml) 1504 6502 26206 1649 6732 27351 Standard deviation 102.27 436.7%3 1205.47 113.78 484.7 1723.11 Standard deviation 102.27 436.7%3 1205.47 113.78 484.7 1723.11 CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3% CV (%) 6.8% 6.7% 4.6% 6.9% 7.2% 6.3%

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity) . Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.029 0.098 0.138 0.233 0.483 0.729 1.27 2.094 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.029 0.098 0.138 0.233 0.483 0.729 1.27 2.094 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.029 0.098 0.138 0.233 0.483 0.729 1.27 2.094 O.D. 0.029 0.098 0.138 0.233 0.483 0.729 1.27 2.094

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.