Human Lithostathine-1-Beta, REG1B ELISA Kit

Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells, and could be associated with fibrocalculous pancreatopathy. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases. Regenerating islet-derived 1 beta (REG1B), also known as Lithostathine-1-beta and Pancreatic stone protein 2 (PSPS2), is a types I Reg protein and contains one typical C-type lectin domain. REG1B is a 166-amino acid protein that has 22 amino acid substitutions in comparison with the previously isolated human REG1A, and it is was expressed only in pancreas. REG1B Is normally found in the exocrine pancreas, whereas in other tissues it appears either only under pathological conditions, such as Alzheimer's disease (brain), cancer (colon), or during regeneration such as neuronal sprouting in brain and pancreas regeneration. REG1B might act as an inhibitor of spontaneous calcium carbonate precipitation. The REG1A and REG1B gene and proteins could play different roles in the pancreas.

Concentration | O.D. | Average | Corrected ------------------------------------------ 250 pg/ml | 2.51 | 2.636 | 2.528 2.762 125 pg/ml | 1.42 | 1.47 | 1.362 1.52 62.5 pg/ml | 0.751 | 0.793 | 0.685 0.835 31.25 pg/ml | 0.447 | 0.458 | 0.35 0.469 15.625 pg/ml | 0.305 | 0.286 | 0.178 0.266 7.8125 pg/ml | 0.209 | 0.197 | 0.089 0.185 3.90625 pg/ml | 0.164 | 0.155 | 0.047 0.146 0 pg/ml | 0.107 | 0.108 | 0 0.108

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 12.2 | 0.39 | 3.2 Intra-assay | 2 | 20 | 53.2 | 1.5 | 2.9 Intra-assay | 3 | 20 | 190 | 4.6 | 2.4 Inter-assay | 1 | 20 | 12.2 | 0.78 | 6.4 Inter-assay | 2 | 20 | 52.8 | 2.5 | 4.7 Inter-assay | 3 | 20 | 191 | 9.2 | 4.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail